Improved data analysis for the MinION nanopore sequencer
نویسندگان
چکیده
منابع مشابه
A first look at the Oxford Nanopore MinION sequencer.
Oxford Nanopore's third-generation single-molecule sequencing platform promises to decrease costs for reagents and instrumentation. After a 2-year hiatus following the initial announcement, the first devices have been released as part of an early access program. We explore the performance of this platform by resequencing the lambda phage genome, and amplicons from a snake venom gland transcript...
متن کاملResolving plasmid structures in Enterobacteriaceae using the MinION nanopore sequencer: assessment of MinION and MinION/Illumina hybrid data assembly approaches
This study aimed to assess the feasibility of using the Oxford Nanopore Technologies (ONT) MinION long-read sequencer in reconstructing fully closed plasmid sequences from eight Enterobacteriaceae isolates of six different species with plasmid populations of varying complexity. Species represented were Escherichia coli, Klebsiella pneumoniae, Citrobacter freundii, Enterobacter cloacae, Serratia...
متن کاملDetecting DNA Methylation using the Oxford Nanopore Technologies MinION sequencer
Affiliations: 1 Ontario Institute for Cancer Research, Toronto, Canada 2 Department of Computer Science, University of Toronto, Toronto, Canada 3 Department of Biomedical Engineering, Johns Hopkins University, Baltimore, Maryland Correspondence to: [email protected] [email protected] Abstract Nanopore sequencing instruments measure the change in electric current caused by DNA transi...
متن کاملCorrigendum: Resolving plasmid structures in Enterobacteriaceae using the MinION nanopore sequencer: assessment of MinION and MinION/Illumina hybrid data assembly approaches
Received 27 July 2017; Accepted 27 July 2017 Author affiliations: 1 Nuffield Department of Clinical Medicine, University of Oxford, Oxford, UK; 2 Division of Infectious Diseases and International Health, Department of Medicine, University of Virginia Health System, Charlottesville, Virginia, USA; 3 The Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford, UK; 4 Addenbrookes Ho...
متن کاملA Novel Diagnostic Method for Malaria Using Loop Mediated Isothermal Amplification (LAMP) and MinION Nanopore Sequencer
BACKGROUND A simple and accurate molecular diagnostic method for malaria is urgently needed due to the limitations of conventional microscopic examination. In this study, we demonstrate a new diagnostic procedure for human malaria using loop mediated isothermal amplification (LAMP) and the MinION™ nanopore sequencer. METHODS We generated specific LAMP primers targeting the 18S-rRNA gene of al...
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ژورنال
عنوان ژورنال: Nature Methods
سال: 2015
ISSN: 1548-7091,1548-7105
DOI: 10.1038/nmeth.3290